With the help of various plugins and apps, you can also add features such as file syncing, encryption, and remote access to your personal cloud server. You can use software such as Nextcloud or ownCloud to create a cloud storage platform for your files. Once you have the components, you’ll need to install the software on your Raspberry Pi and configure it as a server. You can also use a pre-made kit or build it from scratch using these components. To build a personal cloud server with Raspberry Pi, you’ll need a Raspberry Pi board, an external hard drive or USB stick for storage, and some software. With its low cost and small size, Raspberry Pi can be used to build a reliable and secure personal cloud server. If you’re looking to set up a personal cloud server for storing and accessing your files from anywhere, Raspberry Pi is a great platform to start with. So, start building your own portable gaming system with Raspberry Pi today and enjoy gaming on the go! Build a Personal Cloud Server With its low cost and versatility, Raspberry Pi is an accessible platform for anyone looking to get started with gaming projects. This can make it a highly personalized and fun addition to your gaming setup.Ĭreating a portable gaming system with Raspberry Pi is a fun and exciting DIY project that can provide hours of entertainment. With the help of various plugins and mods, you can also add features such as multiplayer support, custom controls, and even voice chat to your portable gaming system. You can then use a gaming emulator such as RetroPie to play classic games from various consoles. Once you have the components, you’ll need to install the software on your Raspberry Pi and set up the display and controllers. To build a portable gaming system with Raspberry Pi, you’ll need a Raspberry Pi board, a display, a battery, and some gaming controllers. With its small size and low power consumption, Raspberry Pi is an ideal platform for building a portable gaming system. If you’re a gaming enthusiast, creating a portable gaming system with Raspberry Pi is a great way to play your favorite games on the go. This can make it a highly personalized and useful addition to your home. With the help of various plugins and APIs, you can also add features such as voice control, facial recognition, and social media integration to your smart mirror. You can then use a programming language such as Python to create a custom interface for your smart mirror. Once you have the components, you’ll need to install the software on your Raspberry Pi and set up the display behind the two-way mirror. You can either buy a pre-made kit or build it from scratch using these components. To build a smart mirror using Raspberry Pi, you’ll need a two-way mirror, a display, a Raspberry Pi board, and some software. A smart mirror is a regular mirror that has a built-in display behind it, which can be used to show information such as the time, weather, and news. If you’re looking for a fun and practical DIY project, building a smart mirror with Raspberry Pi is a great option. So, start exploring the world of home automation with Raspberry Pi and take your home to the next level! Build Your Own Smart Mirror With its low cost and versatility, Raspberry Pi is an accessible platform for anyone looking to get started with DIY smart home projects. Home automation with Raspberry Pi is a great way to make your home smarter and more efficient. You can also use a voice-activated assistant such as Google Assistant or Alexa to control your home appliances using Raspberry Pi. For example, you can use sensors to detect motion in a room and turn on the lights automatically. With the help of a programming language such as Python, you can then write scripts to control the various components and automate tasks. Once you have these components, you can start by setting up your Raspberry Pi and installing the necessary software. To get started with home automation using Raspberry Pi, you’ll need a few components such as a Raspberry Pi board, a relay module, and sensors. With the help of Raspberry Pi, you can automate various tasks in your home such as controlling the lights, monitoring temperature, and even setting up a voice-activated assistant. If you’re looking to upgrade your home with some smart technology, Raspberry Pi is a great platform to start with. If you’re looking for some inspiration for your next Raspberry Pi project, then you’re in the right place! In this article, we’ll be sharing some cool Raspberry Pi projects that you can build at home. Its versatility and accessibility have made it a go-to platform for building innovative projects that range from simple gadgets to complex systems. Raspberry Pi is a low-cost, single-board computer that has captured the hearts of DIY enthusiasts, hobbyists, and students alike.
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11/20/2023 0 Comments Retro alarm clock sonyWhile there are plenty of fun and helpful features of the Jall, one of the most striking aspects is its design. You can toggle the dimmable display between showing temperature or the date as well as a “Sound Control” mode, which turns off the display and automatically turns it back on when movement or sound is detected. Speaking of extra features, this clock can show you the humidity and temperature of your room, which are actually critical factors to getting restful sleep. You can also choose if you want the alarms to go off every day or on weekdays only, which was another pleasant feature that was absent from many of the Jall’s competitors. Whenever you set or change your alarm, you have the option to set two other alarms as well, so you can have three different alarms active at once. The scroll wheel was especially useful, allowing you to speed through the numbers for hours and minutes instead of repeatedly pressing a button. Setting the time and alarm took us less than a minute when we first plugged it in, which was refreshingly short compared to other clocks. To set the time or alarm, all you have to do is press and hold a button and use the wheel to change the numbers on the screen. The Jall clock has a panel on the backside that houses all the controls, including three small buttons, four switches and a scrolling wheel. It’s got a 5-foot cord that should reach most outlets comfortably, and comes with a CR2032 battery that backs up your settings (although the small plastic latch that opens the battery door snapped off when we pulled too hard, so be careful when you need to switch the battery). Right out of the box, this clock was easy to use and setup was a breeze. Plus, at $25 (or less), the Jall Wooden Alarm Clock is equipped with incredibly useful features, such as the ability to set multiple alarms, humidity and temperature readings and a feature that puts the display to sleep and automatically wakes it back up if it senses movement or sound. It’s got a polished feel to it, and its wooden design gives it a look unlike any other clocks we tested. It’s simple to set, easy to read in the dark and has an alarm that is loud but won’t scare you awake. The Jall Wooden Digital Alarm Clock has everything you need - and more - in an alarm clock. Your CNN account Log in to your CNN account 11/20/2023 0 Comments Export file list to text command lineIf you don't like command-line tools, but want a similar program that's a bit more user-friendly, try FileList Creator by Stefan Trost.Īm I the only one that wishes that MS would instead focus on fixing some of the more glaring issues with their software before implementing silly new gimmicks like this? Filelist allows you to use multiple parameters at the same time, and the program will include the additional data in the Output file. For the full list of supported commands, please refer to the official documentation. Similarly, you can add other parameters per your requirements. That may sound like a huge number, but convert it from Bytes to Megabytes and that's just 5 MB. You will need to define the size limit in bytes, so if you want to filter content that's less than 5 MB, the command will be as follows.įilelist C:\Downloads\ /MINSIZE 5000000 > C:\Downloads\example.CSV Maybe you want to exclude some files from the list, for example, those which are less than a particular file size, in which case you can use the MINSIZE option. It is important to note that the parameters should be included before you add the path of the directory, otherwise the program ignores the setting and creates the list with the default rules. Secondly, it discards the last modified, last accessed, and creation date properties. So this command does quite a few things, firstly it includes 2 new columns for the MD5 and SHA256 checksum values of the files. Filelist /USECOLUMNS NAME,EXTENSION,SIZE,FULLPATH,MD5,SHA256 C:\Downloads\ > C:\Downloads\filelist.CSV Say you want to customize the columns that are created by the program, you can use the /USE COLUMNS command to define the format.Į.g. Don't like the default format of the list? FileList supports many parameters, which you can use to include additional properties. Open the document in Microsoft Office Excel or Google Sheets, and it should look much more accessible, with a column for each property. Filelist C:\Downloads\ > C:\Downloads\list.CSV That's precisely what I wanted to highlight, and why you should consider exporting the list to a CSV spreadsheet instead.Īll you have to do is replace the letters TXT in the above command, with CSV.Į.g. Filelist C:\Downloads\ > C:\Downloads\filelist.txtĭon't forget to include the greater than symbol, that's what tells Filelist to save the data as a document.īut wait, even the text document has the same formatting. The syntax for which is Filelist.exe PATH > OUTPUT FILEĮ.g. You can export it to a text document by using a simple command. The formatting makes it difficult to read the list as is in a terminal. In addition to the name of the file, the program also displays other properties such as the file size, last modified, last accessed, creation date, extension and containing path.Īs you can see, Filelist crams a lot of information in the window. This command creates a list of the files that are located in the Music directory. Just type the word Filelist.exe followed by the full path of the directory that you want to scan. The format that you need to use for obtaining the list of files is simple. Open a command prompt window, preferably in the same directory, so it's easier to type the command. to put on a backup volume along with the backed up content.įor those who aren't used to command-line tools, don't let it scare you, the program is pretty easy to use.įileList is a portable application, extract it to a folder of your choice. The command-line utility provides a good way to create a record of your files and folders, e.g. FileList is a freeware tool from JAM Software, the developer of TreeSize. To import our saved books.sql file created earlier, we’d use many of the same flags and much the same syntax.Cataloging the content of the files can help you further understand what's stored where, sort of like a library record. In most cases, importing is just a matter of passing virtually identical options to the mysqlimport command. Now that you’ve learned how to export a backup of a MySQL database, we’ll explore how to reverse the process and import the backup into an existing database.Īs you might imagine, to compliment the mysqldump command used for exporting, there is a similar mysqlimport command for importing. If you need the ability to export (and later recreate) one more more databases, read up on the -databases flag in the official documentation. Instead, by default, only actual tables (and their respective data) are saved and thus will be prepared for later import using this file. sql suffix (which is completely optional but advisable) in the appropriate directory.īy default, mysqldump will not save commands which attempt to modify the existence of the actual database. $ mysqldump -u book_admin -p books > ~/backup/database/books.sqlĪfter entering our password when prompted above, this command then creates our backup file with a. 11/20/2023 0 Comments Superliminal constellations
11/20/2023 0 Comments Anime bdsm tumblrMaybe two people in bed without some sort of harness or metal handcuffs isn’t what modern audiences want to see. Maybe consensual, straightforward sex won’t win the desperate battle for gen Z eyeballs. Maybe missionary sex doesn’t grab headlines. But both characters enjoying themselves all the same. A later BDSM storyline aside, the sweaty awkwardness of Marianne and Connell in Normal People holds the closest mirror to how losing your virginity on a school day might actually go. More than a third of women prefer missionary sex over any other position, but we rarely see that on screen – unless it’s used to imply that the sex is boring or forgettable. Others have called out this trend – as one video noted, “you all have turned vanilla into an insult”. TikTok’s “KinkTok” has fostered a community in which shame is eradicated and anything goes – unless of course, you’re into “vanilla” sex. “Vanilla-shaming” has dethroned “kink-shaming”. If anything, stigma has started to be applied to those who prefer things plain. Psychotherapist Steve Pope told the BBC that non-consensual violence during sex has become a silent epidemic, and people do it because they see it as the norm.Īll this can lead to a continuing conflict between what you see and what you personally want. This combination of sensationalised sex in culture and the lacklustre national curriculum could help explain why more than a third of women under the age of 40 have experienced unwanted choking, slapping or gagging. A fifth of British 14- to 18-year-olds have watched porn on multiple occasions. Meanwhile, sex education is failing to even scratch the surface: in one study, nearly half of the students surveyed said they had been taught nothing about sexual pleasure and teenagers told researchers in 2020 that they turn to porn as an educational tool because schools don’t teach them “what to do”. With fetishes and sexual violence playing out on TV and extreme online porn easily accessible, young people are being given a limited view of sexual desires and preferences, skewed by these platforms’ need to shock and entertain. It also leaves certain types of sex under-represented. The sexologist Jenn Gunsaullus told pop culture site Decider in 2018 that these scenes in TV shows should be in service of the characters and storylines: “If there really is no good plotline that’s engaging folks, then, yeah, it’s just going to be like porn.” When a graphic scene is added just to shock or titillate, it lets down the show – and the audience. But it depends what that sex is being used for. There’s nothing inherently wrong with explicit sex on screen – acceptance of a wide range of preferences has been a long time coming. Extreme sex scenes can drum up coverage and viewers in an increasingly crowded market. As the streamers grow, make more original content and jostle for position, they are behind an ever-greater proportion of TV releases. As a cable network, HBO, alongside streaming services, doesn’t have to stick to the same rules on nudity and sexual content as network TV does in the US. From 2011 until its finale in 2019, HBO’s Game of Thrones churned out rape scenes and plotlines featuring incest and its current prequel series, House of the Dragon, has proved equally barbaric.Įuphoria – which showed porn and statutory rape from its first episode – and The Idol both appeared on HBO, too. The past decade has seen a normalisation of kink-driven, extreme and violent sex on TV. 11/20/2023 0 Comments Highland fried brunchThe OG Sliders with my favorite Tillamook Cheddar cheese were juicy and delicious." - Yelp Elite Kristi C Ice Cream "The El Diablo Wings are the perfect marriage of slightly sweet & spicy. You must try the Sheppard's Pie.comfort food as it was meant to be! The happy hour specials are some of the best!" - Yelper Rachael M "The food and the service is always fantastic. Some amazing apps that are half off, must try the wings and curds (won't be disappointed)." - Yelp Elite Kashmir S They have a great beer selection and it's really cheap. "This has become the weekly late night happy hour hang out with the fellas. The service was outstanding- timely, personal and extremely helpful." - Yelper Caitlin B Happy Hour We had the lobster toast, steak tartare, heirloom salad, gnocchi, and brown butter pudding. I love that the atmosphere is like a little french tavern - no frills, good food and good company." - Yelp Elite Lauren M The rolls are AMAZING! Rose on tap is so fun and so good! The lentil salad, artichokes and wild board is just phenomenal. "Each time I've tried something different and haven't been disappointed. Light and fluffy and not dense, like so many that I've had." - Yelp Elite Gary B Fine Dining "This was some of the BEST Ethiopian food I've ever had. I normally get chai with almond milk and it tasted wonderful." - Yelp Elite Cindy D Ethiopian The service is fantastic and their coffee is great. It has big windows and space for studying/working/catching up. Try the fried chicken - almost Momofuku level on its crispiness and moistness and the fried rice is super flavorful." - Yelper Emma Z Coffee "The food is amazing and the service even better. Plan accordingly and wait for the burger to cool and take in the glory before you." - Yelper Christina G Chinese Highlighted on the 2nd page are the stuffed burgers. Oh! Please, one cannot forget the Cajun fries. It is the gold standard I hold all other brunch and breakfast restaurants to." - Yelper David M Burgers "Cute, adorable little breakfast spot! Everything on the menu is done well! I recommend the eggs Benedict or the Mexican breakfast burrito." - Yelper Tanya M Brunch Plus the baked goods are amazing!" - Yelper Catherine M I love walking here and getting a breakfast burrito to start my weekend off right. Garnish, if desired, and serve."This is the place to get the best breakfast in the neighborhood. “It’s a little bit pick-you-up, put-you-down,” Sparrow says.Īdd all ingredients except garnishes to a blender and blend for 10 to 15 seconds. Just be sure to factor in a little recovery time after you consume this creamy, boozy treat. The rum still shines through (Sparrow uses Coruba but says any dark rum will work) and is accompanied by rich, round flavors of coffee, vanilla, and banana. While the drink comes close to milkshake status, there’s more to it than sweetness. “It’s nice to have a good cool-down drink when they need something to mellow out, instead of just giving them a shot of milk.”īut you don’t need to be facing down a plate of spicy chicken to enjoy it. “People have cried at the bar,” Sparrow says. In this context, the dairy-rich Banana Hammock serves as a sort of break-in-case-of-emergency measure. At the Inman Square restaurant, you can order your Fried Chicken “Nashville hot,” with a high-octane ghost pepper rub and a punishingly spicy sauce. Not only does the drink taste as delicious as it sounds-there’s also a practical reason for its presence on the menu at Highland Fried. “A pretty good antidote to way-too-spicy food,” is how Highland Fried bartender Heather Sparrow describes the Banana Hammock, a frozen concoction created by mixing banana and vanilla ice creams, Coco López (a canned coconut cream), banana and coffee liqueurs, dark rum, and an entire banana in a blender. 11/19/2023 0 Comments Google speedtest webHaving more than one application consuming camera feed can cause the camera not to show up correctly. Only have a single application open that has access to your camera feed. Significato dei termini: - Ping: il ping rappresenta il tempo per inviare e ricevere. Lo speed test restituisce informazioni su ping, jitter, velocità in download e upload della tua connessione sulla rete Top Quality di WINDTRE. SpeedOf.Me is a broadband speed test that allows you to easily measure your actual Internet speed on all your devices like desktop, mobile, tablet, game console, smart TV, etc. Some webcam models have an "ON/OFF" switch - make sure that if yours has one - it is set to "ON" Step 4. Ookla Best internet speed test overall See at Ookla See at Fast Great internet speed test for streamers See at Fast See at M-Lab M-Lab Internet Speed Test Easy to use internet speed. Eseguendo il test puoi misurare la velocità e la qualità della tua connessione Internet mobile, Fibra o ADSL. Auditing tool to diagnose issues and identify opportunities to improve the user experience of web pages. In case you are using an external webcam, check whether it is compatible with the software you are trying to use. Selengkapnya tentang PageSpeed Insights Yang baru Dokumentasi Pelajari Performa Web Ajukan pertanyaan di Stack Overflow Milis. This includes both your operating system, webcam drivers, and software that you use it with. Situs ini menggunakan cookie dari Google untuk memberikan layanannya dan menganalisis traffic. Make sure that all of your software is up to date. For Windows, navigate to the Control Panel, and select 'View Devices and Printers' to see current devices. It runs your web page through an audit for performance, but it also has. For Mac users, you can check hardware status at: About This Mac → System Report → Devices. They usually send us a report they pulled from Google Lighthouse or Pagespeed. When plugging in a new accessory like the webcam, your computer typically will notify you through a small audio signal or an on screen notification. If you are using an external webcam, double check that it is plugged in via USB socket and that its connection cable is not damaged. In addition you could check internet speed with a network speed test as well to compare wireless and wired connectivity.Is your webcam not working? Are you not seeing an image or any information? No problem, just follow these simple steps and we'll get your webcam working in a jiffy: Step 1. You can run a WiFi speed test on all your devices to idenitify the ones that have a strong WiFi signal and the ones that don't. Selectively enabling and disabling your WiFi hotspots can quickly help identify the problematic device. Click Allow so that we can test your webcam. There will be a pop-up on your screen asking for permission. Ookla’s bandwidth diagnostic software shows up on many other speed test sites listed here, but the most full-featured iteration of the test is on, which is owned. Maybe your device is not connecting to the correct WiFi hotspot, so it must go through a thick wall. After you click this button, the test should start. First, we spin-up a browser of your choice: Chrome or Edge. The second section Google PageSpeed simulation is an estimation of your. View important loading events with Googles Core Web Vitals or W3C navigation timing metrics. If the interet speeds differ on your connected devices, however, the poor connectivity is likely either due to a local misconfiguration or device capabilities. The first section Real visitor experience shows the Core Web Vitals of your. If the internet speeds are the same for the weak and strong signal, it's likely an ISP issue. Just run multiple tests, one close to your WiFi Hotspot and one in the place where you're having issues because the signal strength diminishes. So to figure out if your connectivity issues are due to your ISP, your wireless connection or a local router issue running a WiFi Test will give you the insights you're looking for. Test your Internet, download, Facebook Speed with this lightweight Test tool. Most of the devices on your home network today are not connected with a wired connection anymore. PageSpeed Insights (PSI) reports on the user experience of a page on both mobile and desktop devices, and provides suggestions on how that page may be improved. Running a WiFi Speed Test is the right solution for most people if they encounter connectivity issues as they are usually internet speed related. 11/19/2023 0 Comments Gorilla tape radiator hose repairThe adhesive layer on the Gorilla Waterproof Patch & Seal Tape is stronger and thicker, allowing it to hold to surfaces and seal out air, water, and moisture. Not all Gorilla tape is waterproof, but some types, like Gorilla Waterproof Patch & Seal Tape are. If you do not see any bubbles coming from the location of the leak, it means you have repaired the leak correctly and securely, and the hot tub is now safe for use. Test the patchĪfter you have inflated your hot tub, spray the outside of the tub with the water once more. You’ll want to give the tape a few minutes to properly stick before you inflate it again. Be sure not to put any pressure on the tape until it is in the right place. Your aim is to avoid creases as not only will these look unsightly and feel uncomfortable, but they could leat air through. Press firmly over the tape to ensure that it’s stuck tightly. Stick the tape over the holeĬut out a section of tape that’s a few inches larger than the hole on each side and stick it in place. This is to make sure that the tape adheres to the hot tub properly. Once the hot tub is completely deflated, wipe the punctured part with a dry cloth or a paper towel until it’s completely dry. Deflate the hot tubĪfter locating the leak in your blow-up lazy spa, you should let all the air out to allow you to fix it. If the leak is on the outside or top of the hot tub, you won’t see the air escaping so you’ll need to spray or pour water over it until you find where the air is coming from. If it’s on the inside, it should be easy to spot. The location of the leak will produce bubbles. How To Repair an Inflatable Hot Tub Using a Gorilla Tape 1. So be sure to check which kind of Gorilla tape you have and be sure to buy the right one if you need to. These aren’t suitable for repairing your hot tub. Gorilla also makes other kinds of tapes including packaging tape, shipping tape and mounting tape. While regular black or white Gorilla tape, All Weather Gorilla tape, Tough & Wide Gorilla tape or even Crystal Clear Gorilla tape will all work, the Patch & Seal Gorilla tape is most suited to the job of repairing a punctured hot tub. While they’ll all do an okay job of repairing a hot tub leak, the one that you want to buy is Gorilla Waterproof Patch & Seal Tape. There are several types of Gorilla tape available to buy. The Best Type of Gorilla Tape For Hot Tub Repair So if you’re looking for the best glue for hot tub repair, you might want to use Gorilla tape instead. In fact, Gorilla tape is better than many specialist hot tub adhesives as it’s much less messy and faster drying than liquid glue. It has an ultra-strong adhesive layer, making it popular with professional tradespeople as well as DIY hot tub repairs. Gorilla tape is suitable for use in cold, hot, wet, and dry environments. It can be folded and stretched to fit surfaces before application, making it ideal for instant emergency hot tub leak repair. This flexible tape forms a permanent bond thanks to its extra thick adhesive coating and ultraviolet-resistant backing. As stated on the packaging and confirmed in my own tests – this tape seals out water, moisture, and air in an instant. Gorilla tape is made with a highly concentrated rubber-based adhesive and is two to three times thicker than ordinary duct tape. 11/19/2023 0 Comments Hp specter x360 14Regardless, you get 40Gbps of bandwidth, which is great for connecting peripherals. Thunderbolt 4 essentially takes the maximum capabilities of Thunderbolt 3 and makes them standard, but HP already typically used the maximum Thunderbolt 3 configuration. Something else that these new processors enable is support for Thunderbolt 4, but that's not necessarily much different. Best in Class (Image: Future) TechRadar Verdict The HP Spectre x360 (2021) presents a brilliant update on an already excellent laptop, bringing the latest Intel hardware to a stunningly. This integrated GPU is much more powerful than previous generations, and it makes certain GPU-focused tasks easier. These new processors bring a ton of improvements to the table, improving on Intel's 10nm processor architecture, but also include the new Intel Iris Xe graphics. Intel 11th-generation processorsĪs you'd expect with any generational refresh, the latest models of the Spectre x360 family all come with Intel's 11th-generation "Tiger Lake" processors. Shop Amazon for HP Spectre x360 Luxury 14T,13.5' 3:2 WUXGA+ Touch,Intel i7-1165G7, 16GB RAM, 1TB SSD, Bang&Olufsen Quad Speakers, Fingerprint, Tilt Pen, Nightfall Black, Win 10 Pro, 64GB TechWarehouse Flash Drive and find millions of items, delivered faster than ever. Here's a quick rundown of what the latest models added. The latest models of the HP Spectre x360 come with some notable changes, but they're mostly a spec bump from previous models. Intel Wi-Fi 6E AX210 (2×2), Bluetooth 5.Auto Frame, Lighting correction, beauty mode RESET Sponsored (1) HP - Spectre x360 2-in-1 13.5 3K2K Touch-Screen Laptop - Intel Evo Core i7 - 16GB Memory - 1TB SSD - Nightfall Black 14-ef0013dx Tablet.16 inch AMOLED, 4K (3840 x 2400), anti-reflection, touch.16 inch IPS, 3K+ (3072 x 1920), touch, optional anti-reflection coating.13.3 inch IPS, Full HD (1920 x 1080), HP Sure View, touch.Up to Intel Core i7-11390H (up to 5GHz, 4-core). 11/19/2023 0 Comments False negativeMutation calls from two cell-line databases including GDSC (Genomics of Drug Sensitivity in Cancer) and CCLE (Cancer Cell Line Encyclopedia) were constructed by whole-exome- or equivalently highly-multiplex NGS inconsistent mutation calls have been reported to be as high as 43%, suggesting a possible high rate of error in NGS. To establish any firm regulatory rules, further studies on the reproducibility or reliability of NGS results are needed.įor NGS analyses, target enrichment, amplification, sequencing processes, and bioinformatics analyses are involved, but the estimation of NGS errors have not been comprehensive. The Food and Drug Administration (FDA) has finalized a guidance document to accelerate the establishment of a regulatory approach for NGS testing, though the actual regulations are still debated. Currently, in the USA, the clinical laboratories for NGS testing are overseen by the Centers for Medicare and Medicaid Services (CMS) according to the Clinical Laboratory Improvement Amendments (CLIA) regulations. Some of the errors seem to be related to the NGS instrumentation itself, the SOLiD platform having shown an erroneous variant calling rate as high as 20–40% in a study. In a study with 20,000 samples, the NGS false-positive rate was 1.3%, the authors suggesting that Sanger confirmation is required for NGS panel testing. Despite widespread adoption of NGS technology for the purposes of clinical diagnosis, high error rates on various NGS platforms has been reported (0.26–12.86%). According to the American Clinical Laboratory Association (ACLA), over 11,000 laboratories and companies in the USA have developed their own NGS tests. Whereas classical genetic tests measure limited base changes or structural changes in DNA, recently introduced next-generation sequencing (NGS) technology can examine millions of DNA variants at a time. Whole-exome-next-generation sequencing TP, H.Ĭompeting interests: The authors have declared that no competing interests exist. All other data are available in supplementary tables.įunding: This study was supported by grants from the National Cancer Center (NCC), Korea (1510121, 1831130, 1811030, and 1910150), and a grant from the National Research Foundation, Korea (NRF-2015R1A2A2A04007432) to K-M. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: MAF files from our targeted NGS in 35 cell lines are available at as described in Materials and Methods. Received: JAccepted: AugPublished: September 12, 2019Ĭopyright: © 2019 Kim et al. PLoS ONE 14(9):Įditor: Amanda Ewart Toland, Ohio State University Wexner Medical Center, UNITED STATES (2019) False-negative errors in next-generation sequencing contribute substantially to inconsistency of mutation databases. Citation: Kim Y-H, Song Y, Kim J-K, Kim T-M, Sim HW, Kim H-L, et al. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |